响应面法优化红莲外皮原花青素的提取工艺研究

Optimization of Extraction of Proanthocyanidins from Skin of Red Locus Seed Using Response Surface Methodology

本实验采用单因素试验和响应面分析法来确定红莲外皮中原花青素提取的最佳工艺条件。通过单因素试验探讨提取溶剂的种类、提取溶剂的浓度、酸的种类、pH、液料比、提取温度、提取时间、提取次数这几个因素对原花青素提取效果的影响;根据单因素试验结果固定提取温度40℃,提取时间90 min,提取次数1次,选择pH、液料比和丙酮浓度进行三因素三水平的响应面试验,依据回归分析得到最优工艺条件为:pH 2.6,液料比55 mL/g,丙酮浓度67%。此工艺条件下红莲外皮原花青素提取率为9.57%。

The extraction of proanthocyanidins from skin of red locus seed were investigated by the single experiments and response surface methodology.The single experiments were used to explore the effects of different extractant,extractant concentration,different acid,pH,liquid-to-material ratio,extraction temperature,extraction time and extraction times on the yield of proanthocyanidins.The best extraction temperature,extraction time and extraction times were determined as 40 ℃,90 min and one time,respectively.Then the extraction pH,liquid-to-material ratio,and acetone concentration were investigated through response surface experiment.Based on the regression analysis,the extractive conditions were optimized as follows： pH 2.6,liquid-to-material ratio 55 mL/g and acetone concentration 67%.The extraction rate of proanthocyanidins of this optimized procedure reached 9.57 %.