酶联免疫法测定苦荞制品中的黄曲霉毒素B_1

Determination of Aflatoxin B_1 in Tartary Buckwheat Products by Enzyme-linked Immunosorbent Assay

用酶联免疫法(ELISA)测定不同苦荞制品中的黄曲霉毒素B1,对如何防控苦荞制品中的黄曲霉毒素B1进行探讨。在分析的4类产品中,苦荞营养粉的黄曲霉毒素B1含量最低,其次是苦荞速溶片和苦荞醋,苦荞米茶中最高。方法的检测灵敏度为0.1μg/kg,平均回收率在84.70-84.95%,相对标准偏差小于5%。结果表明,酶联免疫法测定准确,稳定性和重现性好,可广泛应用于苦荞及其制品中黄曲霉毒素B1的检测。

The content of aflatoxin B1 in tartary buckwheat products was detected with ELISA kit,and the prevention and control measures of aflatoxin B1 were discussed.The aflatoxin B1content of tartary buckwheat nutrition power was the least in the 4 kinds of produces analyzed,followed by tartary buckwheat flast dissolving tablets and tartary buckwheat vinegar.The aflatoxin B1 content of tartary buckwheat tea was the highest.The detection sensitivity was 0.1 μg/kg and the average spike recovery rate was 84.70-84.95%.The relative standard deviation was less than 5%.The results showed that the ELISA was accurate with good stability and reproducibility,so it can be applied to detect aflatoxin B1 in tartary buckwheat products widely.