巢式PCR在弓形虫检测和基因研究中的应用进展

Advances in use of nested PCR to detect and genetically study Toxoplasma gondii

弓形虫是一种专性细胞内寄生的寄生虫,人感染后多呈隐性感染,临床检测及诊断困难。巢式PCR是利用2套引物,对靶细胞基因2次识别并进行2轮扩增的方法,它可从极少量的模板中获得较高浓度和纯度的靶片段,在弓形虫的检测和基因研究中具有敏感性高、特异性强、稳定性好的优点。本文对巢式PCR在弓形虫中的应用进行了综述。

Toxoplasrna gondii is an obligate intracellular parasite that is difficult to detect and diagnose clinically in people who are infected. Nested PCR uses two sets of primers to amplify genes of target cells twice to identify them, resulting in a higher concentration and purity of target fragments from a tiny amount of template. When detecting T. gon dii, nested PCR also has advantages such as sensitivity, specificity, and consistency. This paper reviews advances in the use of nested PCR to detect and genetically study T. gondii.