乙型肝炎病毒外膜大蛋白含量与核酸载量的相关性研究

The correlation research between the content of HBV large envelope protein ( HBV-LP) and the HBV-DNA load in hepatitis B

目的探讨乙型肝炎患者外膜大蛋白(HBV-LP)含量与HBV-DNA的相关性及临床意义。方法对270例HBV感染者采用酶联免疫吸附试验(ELISA)检测血清的HBV-LP、HBeAg,实时荧光定量PCR法定量检测HBV DNA,并进行相关性分析。结果 270例HBV感染者血清中HBV-LP含量与HBV DNA拷贝数变化相一致,两者呈正相关;在152份HBeAg阳性血清中,HBV DNA与HBV-LP的阳性率分别为95.142%和93.189%,两者差异无显著性;在118份HBeAg阴性血清中,HBV DNA与HBV-LP的阳性率分别为46.102%和56.125%,两者差异无显著性。结论血清HBV–LP含量能反映HBV感染者体内HBV复制程度,其灵敏度高于HBeAg,可作为判断HBV复制新的血清学指标。

Objective This paper explores the correlation and the clinical value between the content of HBV large envelope protein （ HBV-LP） and the HBV-DNA load in hepatitis B patients. Methods 270 patients underwent ELISA for serum HBV-LP and HBeAg,and HBV-DNA was quantitatively detected by using real time polymerase chain reaction. Results In 270 serum samples,the level of HBV-LP and HBV-DNA copies performed a nice positive correlation of the patient with HBV infection; In 152 HBeAg positive serum samples,no significant difference of positive rate was found between HBV-LP （ 93. 189% ） and HBV-DNA （ 95. 142% ） ; In 118 HBeAg negative serum samples, no significant difference of positive rate was found between HBV-LP （ 56. 125%） and HBV-DNA （ 46. 102%） . Conclusion Serum HBV-LP can reflect hepatitis B virus replication level,and the sensitivity is higher than HBeAg,it can be a new index of hepatitis B virus replication.