大豆结荚习性、荚色和种皮色相关野生片段分析

Identification of Wild Segments Associated with Stem Termination,Pod Color,and Seed Coat Color in Soybean

结荚习性、荚色和种皮色是大豆的重要形态性状,与进化密切相关。利用由151个家系组成的野生大豆(Glycine soja Sieb et Zucc.)染色体片段代换系(CSSL)群体(SojaCSSLP1),通过不同表型CSSL组间比对,分别检测到与结荚习性、荚色和种皮色相关的1、3和2个野生片段(基因)。其中,5个野生片段(基因)分别与前人在栽培豆中检测到的无限结荚Dt1、荚色L1、荚色L2、绿种皮G和黑种皮i基因相对应,说明野生大豆与栽培大豆间、栽培大豆与栽培大豆间在这些片段上均存在等位变异分化,是与大豆进化相关的基因/片段。另一个与荚色相关的Satt273野生片段能使大豆表现黑荚,可能是本研究的新发现,但还需进一步验证。

Stem termination （ST）, pod color （PC） and seed coat color （SCC） are important morphological traits, which are related to evolution in soybean. By using a wild soybean （Glycine soja Sieb et Zucc.） chromosome segment substitution line （CSSL） population, designated as SojaCSSLP 1 composed of 151 lines, one ST, three PC and two SCC wild segments/alleles were detected based on the comparison of different CSSL groups with a same phenotype on the respective trait. Among them, five wild al- leles/segments identified in this study were corresponding to Dtl, L2, L1, G and i, respectively, which indicated that there existed allele differentiation happened between wild and cultivated soybean as well as between cultivated soybeans on these loci/segments and that the genes/segments involved with domestication and evolution. The wild segment of Satt273 for PC might be a novel gene/segment, which needed further verification. The identification of the genes/segments provide basic materials for cloning the wild alleles, studying the wild allele function and recombination of the wild alleles/segments.