CdTe量子点与人血清白蛋白的相互作用研究

Studies on Interaction between CdTe Quantum Dots and Human Serum Albumin

以水相合成法制备了谷胱甘肽(GSH)修饰的CdTe量子点,并运用紫外-可见吸收光谱(UV-vis)、荧光发射光谱(FL)以及X射线衍射(XRD)对合成所得量子点进行了表征。采用荧光光谱、圆二色谱以及紫外-可见吸收光谱等方法,在近生理条件下研究了谷胱甘肽修饰CdTe量子点与人血清白蛋白(HSA)的相互作用。荧光猝灭和紫外-可见吸收光谱试验结果表明,人血清白蛋白(HSA)和CdTe量子点形成了静态复合物,相互作用力以静电引力为主,而且CdTe量子点会引起HSA构象发生变化。研究结果可为纳米材料的生物安全性研究提供参考。

CdTe quantum dots(QDs) modified with glutathione(GSH) are prepared by using aqueous synthesis method.The structures and optical properties of the obtained CdTe QDs are characterized by UV-vis absorption spectroscopy,photoluminescence spectroscopy and X-ray diffraction.The interaction between human serum albumin(HSA) and CdTe QDs capped with GSH are investigated under physiological conditions.The investigation is carried out by using fluorescence spectroscopy,circular dichroism(CD) spectra and UV-vis spectroscopy.The results of fluorescence quenching and UVvis absorption spectra experiments indicate that the formation of complex HSA and GSH-CdTe QDs.The electrostatic interactions play predominant roles in the adsorption process.Furthermore,it is also proven that QDs can induce the conformational change of HSA.The research results provide important theoreticdata for evaluation of QDs.