三七和栀子有效成分对APP转基因小鼠脑内淀粉样蛋白的作用

Experiment effect of the active ingredients of Sanchi and Gardenia on the amyloid in the APP transgenic mouse brain

目的:研究三七和栀子有效成分对APP转基因小鼠脑内淀粉样蛋白的影响。方法:转基因小鼠雌雄各半,分别随机分为三七和栀子有效成分高剂量组、中剂量组、低剂量组,阳性药安里申组,模型组。同窝出生的野生型小鼠作为阴性对照组。小鼠自6月龄开始自主进食给药4个月,然后进行免疫组织化学、Western Blot、ELISA实验,检测海马和皮层区淀粉样蛋白Aβ1-40、Aβ1-42及APP的表达水平和脑内的淀粉样斑块。结果:在雌性小鼠各组中,三七和栀子有效成分低剂量组能够显著性降低海马区Aβ1-40和Aβ1-42的水平,且淀粉样蛋白前体蛋白APP水平显著性降低。结论:三七和栀子有效成分可能作用于APP生成阶段,通过下调APP的产生,从上游降低了Aβ产生的风险。

Objective： To investigate the effect of the active ingredients from Sanchi and Gardenia on β-amyloid protein of the APP transgenic mouse brain. Methods： Transgenic mice were divided into halves： male and female. And each group was randomly divided into high dose, middle dose, low dose of herb treatment groups, positive control and model groups. Negative control group contain wild-type mice which are littermates of transgenic ones. The expression levels of Aβ1-40, Aβ1-42, APP in hippocampus and cortex were detected by Western Blot and ELISA tests, while amyloid plaques in brain were examined using immunohistochemistry methods. Results： In female group, Sanchi and Gardenia low dose group shows significantly reduced levels of Aβ1-40, Aβ1-42 in hippocampus as well as APP protein. Conclusion： The active ingredients of Sanchi and Gardenia may have the effect on the expression level of APP, reducing the risk of Aβ production from the upstream by down-regulating the expression level of APP.