富勒烯修饰电极微分脉冲伏安法测定盐酸克伦特罗

Differential Pulse Voltammetric Determination of Clenbuterol with Fullerene Modified Electrode

将1.08mg富勒烯(C60)分散在10mL二氯甲烷中,取40μL悬浮液滴涂在玻碳电极表面,吹干后在1mol·L-1氢氧化钾溶液中进行活化,制得C60修饰电极。用循环伏安法研究了修饰电极的电化学行为。结果表明:经氢氧化钾处理后的修饰电极,在5mmol·L-1铁氰化钾溶液中,可见一对准可逆的氧化还原峰,且峰电流显著高于在裸玻碳电极和未处理电极上的峰电流。据此提出了用微分脉冲伏安法测定盐酸克伦特罗的方法。盐酸克伦特罗的浓度在0.1～20μmol·L-1范围内与其氧化峰电流呈线性关系,检出限(3S/N)为3.26×10-2μmol·L-1。方法用于尿样分析,回收率在99.5%～110%之间。

Fullerene（C60,1.08 mg） was dispersed in CH2Cl2（10 mL）,and dropped on the surface of glassy carbon electrode（GCE）,then activated in 1 mol·L-1 KOH solution to prepare C60 modified GCE（C60/GCE）.Electrochemical behavior of the modified electrode was studied by cyclic voltammetry.As shown by the experimental results,a pair of quasi-reversible oxidation and reduction peaks was observed in 5 mmol·L-1 K3Fe（CN）3 solution at the modified electrode,and the peak currents were found to be higher than those obtained at bare GCE and unactivated modified electrode.Based on these findings,a selective method for determination of clenbuterol（CLB） by differential pulse voltammetry was proposed.Linear relationship between values of oxidation peak current and concentration of CLB was kept in the range of 0.1-20 μmol·L-1 with detection limit（3S/N） of 3.26×10-2μmol·L-1.The proposed method was used in the analysis of samples of urine,and values of recovery found were in the range of 99.5%-110%.