摘要
目的观察复发性生殖器疱疹(recurrentgenital herpes,RGH)患者血清白细胞介素2(interleukin-2,IL-2)水平及外周血T淋巴细胞亚群的变化规律,探讨RGH患者反复发作的免疫学机制。方法选择100例RGH患者,采用酶联免疫吸附测定方法检测IL-2,采用流式细胞仪检测T淋巴细胞亚群。对照组100例来自无感染性疾病的健康志愿者。结果 RGH组血清IL-2水平明显低于正常对照组(P<0.01)。RGH组外周血清中的CD3^+、CD4^+、CD4^+/CD8^+ T细胞表达较对照组明显降低(P<0.05);而CD8^+T细胞表达则明显高于对照组,差异有统计学意义(P<0.05)。RGH组老年患者外周血CD3^+、CD4^+、CD4^+/CD8^+T细胞明显低于青年患者(P<0.05),而CD8^+ T细胞明显高于青年患者(P<0.05)。结论 RGH患者血清中IL-2水平及外周血T淋巴细胞亚群水平异常,患者体内存在细胞免疫功能失调及免疫抑制现象。
Objective To detect the expression of interleukin-2 (IL-2) and T lymphocyte subsets in peripheral blood of patients with recurrent genital herpes ( RGH ), and to discuss the immunological mechanism of repeated recurrent genital herpes. Methods One hundred cases of RGH patients were selected as research objects. IL-2 levels were detected by enzyme linked immunosorbent assay (ELISA), and the T lymphocyte subsets were detected by flow cytometry. Another 100 healthy volunteers without infectious diseases served in control group. Results The serum IL-2 level of RGH group was significantly lower than that of normal control group ( P 〈 0.01 ) ; Peripheral serum CD3 + , CD4 + , CD4 +/CD8 +T expression in RGH group were significantly lower than those of the control group (P 〈 0.05 ) ;while the CD8 +T cell expression was significantly higher than that of the control group( P 〈 0.05 );peripheral blood CD3 +, CD4 +, CD4 +/CD8 +T ceils of elderly patients in RGH group was significantly lower than that of young patients (P 〈 0.05 ), and CD8 + T cells was significantly higher than that of young patients (P 〈 0.05 ). Conclusion The IL-2 and T lymphocyte subsets of patients with RGH were abnormal. There are cellular immune dysfunction and immune suppression in the patients with RGH.
出处
《河北医科大学学报》
CAS
2013年第6期664-666,共3页
Journal of Hebei Medical University