中国黄羽鹌鹑消化道生长抑素细胞的免疫组织化学研究

Immunohistochemistry of somatostatin endocrine cells in digestive tract of Chinese Yellow Quail(Coturnix aponica)

【目的】研究中国黄羽鹌鹑消化道生长抑素(Somatostatin,SS)细胞的分布及形态学特点。【方法】采集中国黄羽鹌鹑的食管、嗉囊、腺胃、肌胃、十二指肠、空肠、回肠、盲肠及直肠,常规方法制备石蜡切片,用链霉亲和素-生物素-过氧化物酶复合物(streptavidin-boin-peroxidase complex,SABC)免疫组织化学方法进行SS细胞定位。【结果】SS细胞主要分布于腺胃及小肠各部位,其中以腺胃分布数量最高,十二指肠次之,空肠、回肠及直肠分布均较少;食管、嗉囊、肌胃及盲肠处均未发现有其分布。统计分析结果表明,腺胃SS细胞的分布数量显著高于肠道各部(P<0.05),而肠道各部位间不具有显著性差异(P>0.05)。SS细胞的形态以圆形、椭圆形、三角锥形及梭形为主,多分布于腺胃腺叶内细胞之间、肠腺上皮细胞之间和肠黏膜上皮细胞之间。SS细胞的分布数量在雌雄鹌鹑各相应部位间不具有显著性差异(P>0.05),且形态特征相同。【结论】中国黄羽鹌鹑消化道SS细胞呈现由小肠头端向直肠尾端逐渐减少的分布趋势,在腺胃处分布数量最高,形态多为圆形、椭圆形、三角锥形及梭形,这可能与其消化道相应部位的生理功能有关。

【Objective】 This experiment aimed at studying the distribution and morphology of somatostatin secreting cells in digestive tract of Chinese Yellow Quail（Coturnix aponica）.【Method】 Tissue materials were collected from esophagus,ingluvies,proventriculus,gizzard,duodenum,Jejunum,ileum,caecum and colorectum.The streptavidin-biotin-peroxidase complex（SABC）immunohistochemical method was used for position identification of somatostatin secreting cells.【Result】 Results showed that somatostatin secreting cells were found in proventriculus and small intestines rather than esophagus,ingluvies,gizzard and caecum.The highest distributive part was proventriculus,followed by duodenum.Jejunum,ileum and colorectum had much lower numbers.However,results of statistical analysis showed that there were no significantly difference（P0.05）among the various parts of intestines while intestines and proventriculus had significantly difference（P0.05）.Somatostatin secreting cells were mainly round,oval,triangular pyramidal and fusiform.Many of them could be found between the cells of proventriculus leaves,in the crypt of intestines and between the epithelial cells.Besides,there was no significant difference（P0.05）in the numbers of somatostatin secreting cells between the same parts of female and male,and their shapes were quite the same.【Conclusion】 The distributive curve of the somatostatin secreting cells in the digestive tract of Chinese Yellow Quail was decreasing from the anterior to the posterior intestines.Proventriculus was the highest distributive part.Round,oval,triangular pyramidal and fusiform were the main shapes of Somatostatin secreting cells,which may be relevant to the function of each part in digestive tract.