子宫内膜异位症孕妇女性胎儿HOXA10基因异常甲基化观察及意义

Observation of female fetuses HOXA10 gene aberrant methylation in pregnant women suffering from endometriosis

目的检测子宫内膜异位症孕妇及正常孕妇其女性胎儿脐血子宫内膜异位症易感基因HOXA10甲基化状态,分析内异症的家族遗传性。方法收集15例内异症孕妇及26例正常孕妇女性胎儿的脐血标本(分别为内异症组及正常组)。分别采用甲基化特异性聚合酶链反应(MSP)及亚硫酸氢盐修饰后测序法(BSP)检测两组HOXA10基因的甲基化程度。结果两种方法检测内异症组HOXA10甲基化率均显著高于正常组,P均<0.05。结论从表观遗传学上讲内异症可能具有家族遗传性。

Objective To detect the endometriosis susceptibility gene of HOXA10 promoter region aberrant methylation in pregnant women with endometriosis and normal pregnant women, and to analyze the family heredity of endometriosis. Methods Specimens were collected from January 2010 to December 2012 hospitalized in Zhujiang Hospital of Southern Medical University, which included fifteen female fetus cord blood specimens of pregnant woman with endometriosis and twenty-six female fetus cord blood specimens of normal pregnant woman. The HOXA10 aberrant methylation in two groups were detected by methylation specific polymerase chain reaction （MSP） and bisulfite salt modified sequencing （BSP）. Re- sults The fetal cord blood HOXAIO methylation rate in pregnant women with endometriosis was higher than that in normal pregnant women, with statistically significant difference （all P 〈 0.05）. Conclusion As in the epigenetics, endometrio- sis may have family heredity.