肿瘤侵犯相关蛋白-自分泌运动因子对真皮成纤维细胞生物学特性的影响

Effects of tumor invasion related protein-autocrine motility factor on biological property in human dermal fibroblasts

目的:观察自分泌运动因子(autocrine motility factor,AMF)对体外培养的人真皮成纤维细胞(human dermal fibroblasts,HDF)生物学特性(增殖、抗凋亡和迁移运动)的影响。方法:HDF体外培养后分为AMF组和空白对照组。MTT法检测细胞增殖活性,流式细胞仪检测细胞周期及细胞抗凋亡能力,Transwell小室迁移运动模型检测细胞迁移运动能力。结果:与空白对照组相比,AMF组体外培养的HDF的增殖能力(t=9.13,P<0.001)、抗凋亡能力(t=30.94,P<0.001)及运动迁移能力(t=5.35,P=0.003)均显著增强。结论:AMF具有提高HDF增殖、运动迁移和抗凋亡能力,提示该因子与瘢痕疙瘩的肿瘤学特征有着密切关系。

Objective：To observe the effects of autocrine motility factor（AMF）on the biological property（proliferation,anti-apoptosis and migration）of the cultured human dermal fibroblasts（HDF）.Methods：Cultured HDF were divided into experimental group（AMF group）and blank control group.Cellular anti-apoptosis and cell cycle were analyzed by flow cytometry.Proliferation and migration of the cells were respectively determined by MTT assay and Transwell chamber.Results：Abilities of proliferation（t=9.13,P〈0.001）,anti-apoptosis（t=30.94,P〈0.001）and migration（t=5.35,P=0.003） were increased significantly in experimental group compared with those in blank control group.Conclusions：AMF could induce abilities of proliferation,anti-apoptosis and migration of HDF,suggesting that AMF is associated with keloid in characteristics of oncology.