参附注射液预先给药对内毒素血症大鼠肠黏膜上皮的影响

Effects of Shenfu injection pretreatment on intestinal epithelium of endotoxemia rats

目的:观察参附注射液预先给药对内毒素血症大鼠肠黏膜上皮的影响。方法:48只大鼠随机分为模型组(M组,n=24)和参附注射液预先给药组(S组,n=24),采用脂多糖(10 mg/kg)单次尾静脉注射建立内毒素血症模型。M组和S组大鼠分别于建模前30 min腹腔注射生理盐水和参附注射液5 ml/kg,分别在建模后2、6、12 h随机取8只收集标本。另取8只大鼠作为空白对照组(C组,n=8)。取腹主动脉血,ELISA检测血浆肠脂肪酸结合蛋白(intestinal fatty acid binding protein,i-FABP)和二胺氧化酶(diamine oxidase,DAO)水平变化;取远端回肠,ELISA检测肠黏膜肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)和白细胞介素-6(interleukin-6,IL-6)水平,HE染色观察肠黏膜形态学改变并行Chiu氏评分。结果:与C组比较,M组各时相点大鼠血浆i-FABP、DAO水平、肠黏膜TNF-α、IL-6水平升高,肠黏膜Chiu氏评分增加(P<0.05)。与M组比较,S组2 h时相点大鼠血浆i-FABP、DAO水平、肠黏膜TNF-α、IL-6水平降低,肠黏膜Chiu氏评分降低(P<0.05);S组6 h时相点大鼠肠黏膜TNF-α、IL-6水平降低(P<0.05)。结论:参附注射液预先给药在内毒素血症早期可在一定程度上抑制炎症反应,降低大鼠血浆i-FABP和DAO水平,减少肠黏膜Chiu氏评分,减轻肠黏膜上皮损伤。

Objective：To explore the effects of Shenfu injection pretreatment on intestinal epithelium of endotoxemia rats.Methods： Forty-eight adult male rats were randomly divided into endotoxemia model group（group M,n=24）and Shenfu injection pretreatment group（group S,n=24）.Rats in group M and group S were further divided into 2 h,6 h,12 h subgroups and each subgroup had eight rats.A single caudal venous injection of lipopolysaccharide（10 mg/kg）was used to induce endotoxemia model.Thirty minutes before endotoxemia model was induced,rats in group M and group S respectively intraperitoneally received saline（5 ml/kg）and Shenfu injection（5 ml/kg）and samples were respectively obtained at each time point.Another eight rats did not do any treatment were taken as control group（group C,n=8）and samples was collected directly.Blood was collected through abdominal aorta to measure intestinal fatty acid binding protein（i-FABP）and diamine oxidase（DAO）in serum by ELISA.Distal ileum was collected;levels of tumor necrosis factor-α（TNF-α）and interleukin-6（IL-6）in intestinal mucosa were detected by ELISA;morphological changes were observed by HE for staining and pathological scores were evaluated according to Chiu＇s score.Results：I-FABP and DAO levels in blood plasma,TNF-α and IL-6 levels in intestinal mucosa and Chiu＇s scores in group M were all significantly increased at each time point compared with those in group C（P〈0.05）.At 2 h after endotoxemia model establishment,i-FABP,DAO,TNF-α and IL-6 levels as well as Chiu＇s scores in group S were lower than those in group M（P〈0.05）,meanwhile,TNF-α and IL-6 levels in group S were lower than those in group M（P〈0.05）.Conclusions：Shenfu injection pretreatment on endotoxemia rats could attenuate inflammatory response,decrease serum i-FABP and DAO levels,lower pathological scores and alleviate the injury of intestinal epithelium at early stage.