果糖对电离辐射诱发AHH-1细胞凋亡的保护作用

Radioprotective effects of fructose on irradiation induced apoptosis in AHH-1 cells

目的:观察果糖对淋巴母细胞AHH-1辐射损伤保护作用。方法:照射前12 h给予不同浓度的腺嘌呤,再经4.0 Gy60Coγ射线照射诱发细胞凋亡,采用MTS法计算细胞存活率;Annexin V-FITC/PI双染法检测细胞凋亡率;RT-PCR分析细胞Caspase-9、Bax、Bcl-2 mRNA表达的变化;Western blot分析细胞Caspase-9、Bax、Bcl-2蛋白改变。结果:与正常组相比,电离辐射后细胞存活率78.0%显著下降(P<0.05),细胞照射前加入果糖其存活率随浓度的增加而升高(P<0.05),细胞中Caspase-9、Bax的mRNA和蛋白表达明显减少,Bcl-2的mRNA和蛋白表达明显增加,凋亡的各项指标均明显改善。结论:果糖对电离辐射诱发的细胞凋亡有保护作用,并对细胞凋亡的抑制呈剂量依赖正相关关系。

Objective：To investigate radioprotective effects of fructose on irradiated lymphocytoblast AHH-1 and to discover the possible mechanisms.Methods：Lymphocytoblasts were pretreated for 12 h before radiation with different concentrations of fructose and then were exposed to 4 Gy 60 Coγ ray radiation to induce cell apoptosis.Cell survival rate was counted by MTS assay and cell apoptosis rate was detected by Annexin V/PI.Changes in mRNA expressions of Caspase-9,Bax,Bcl-2 were investigated by RT-PCR and changes in protein expressions of Caspase-9,Bax,Bcl-2 were analyzed by Western blot.Results：Irradiation increased cell death and cell survival rate was 78% after irradiation,significantly lower compared with that before irradiation（P〈0.05）.If fructose was added before irradiation,cell survival rate was increased in a concentration dependent manner after irradiation（P〈0.05）.mRNA and proteins expressions of Caspase-9 and Bax were significantly decreased and mRNA expressions of Bcl-2 were increased;apotososis related indexes were improved.Conclusions：Fructose exerts radioprotective effects on irradiation induced apoptosis in AHH-1 cells and its inhibition on cell apoptosis is in a concentration dependent manner.