高效液相色谱法测定虎杖提取物中白藜芦醇的含量

Determination of erbascoside and resveratrol in root of polygonum cuspidatum by high performance liquid chromatography

目的:建立虎杖提取物中白藜芦醇的含量测定方法。方法:采用高效液相色谱法测定虎杖提取物中白藜芦醇的含量。色谱条件:流动相:乙腈-水(30∶70);流速:1.0 ml/min;检测波长:306 nm;柱温:室温;进样量:10μl。结果:白藜芦醇进样量在0.3～9.6μg/ml范围内,线性关系良好。结论:本法简便实用、灵敏度高、重现性好,可用于虎杖提取物中白藜芦醇的含量测定。

Objective：To establish reserved-phase high performance liquid chromatography（HPLC）method for the determination of resveratrol content in root of polygonum cuspidatum.Methods：HPLC was carried out to detect resveratrol content in root of polygonum cuspidatum.Chromatographic conditions：mobile phase consisting of acetonitrile-water（30∶70）at flow rate of 1.0 ml/min;detection wave length of 306 nm;column temperature at room temperature;sample size of 10 μl.Results：Calibration curves were linear within the range of 0.3~9.6 μg/ml.Conclusions：The method is simple,accurate,reproducible,precise and can be used in the determination of resveratrol content in polygonum cuspidatum extract.