雌二醇浓度对大鼠髁突软骨细胞中雌激素受体mRNA表达的影响

Effect of estradiol on the expression of estrogen receptor in mandibular condylar cartilage cell of rat

目的:探讨雌二醇(estradiol,E2)对体外培养的髁突软骨细胞雌激素受体(estrogen recerptor,ER)基因表达的影响。方法:体外培养大鼠髁突软骨细胞,分别加入浓度为10-12、10-9、10-6、10-3mmol/L的17βE2 24 h,实时定量PCR法检测所培养的髁突软骨细胞ERα、ERβmRNA的表达。采用SPSS10.0软件包对结果进行统计学分析。结果:ERα和ERβ在体外培养的髁突软骨细胞中均有表达,17βE2能够上调ERα的基因表达,同时下调ERβ表达。在10-9mmol/L的17βE2作用下,上调ERα基因表达最为明显。结论:E2浓度的改变可上调ERα的表达并下调ERβ的表达,在生理范围的雌激素浓度下,E2上调ERα基因表达的作用最为明显。

PURPOSE： To evaluate the effect of estradiol on the expressions of estrogen receptor in mandibular condylar cartilage cell （MCC）. METHODS： The MCC were harvested from neonatal rats. The experimental groups was cultured in medium with different concentration （10-3,10-6,10-9,10-2 mmol/L） of estradiol （E2）, while the control group was cultured in medium with ethanol. The ERa mRNA and ERI3 mRNA in cells were detected with RT-PCR.The data was processed with SPSS10.0 software package. RESLUTS： Both ERa and ERI3 expression were observed in MCC. Exogenous 1713-E2 raised the gene expression of ERa and lowered the expression of ERI3. Under the concentration of 10-9 mmol/L （physiological condition）, the up-regulation of ERa mRNA was found most obviously. CONCLUSIONS： E2 can raise the expression of ERa and decrease the expression of ERI3, and the effect of up grading is the most obvious under physiological concentration.