响应面分析法优化红花多糖的提取工艺

Optimization for Extraction of Safflower Polysaccharides by Response Surface Methodology

目的利用响应面分析法对红花多糖提取工艺进行优化。方法在单因素实验基础上选取实验因素与水平,根据中心组合实验设计原理,采用三因素三水平的响应面分析法,以获得多元二次线性回归方程,以多糖提取率为响应值作响应面和等高线。结果确定了水提红花多糖的最佳工艺条件:提取温度94.71℃,提取时间1.67 h,水料比22.04 mL.g-1,提取2次。在此条件下红花多糖提取率理论值达到2.789%,验证实验结果与理论接近。结论该优化工艺的多糖提取率高,可用于红花多糖的提取。

Objective To optimize the extraction of safflower polysaccharides by response surface methodology. Methods Experiment factors and levels were first selected on the ground of one factor test along with the central composite experimental design principles, the response surface methodology with three factors and three levels was adopted in search of multiple quadratic linear regression. Response surface and contour were finally chosen as the extraction rate and the response value respectively. Results The optimum extraction conditions of the polysaccharides from safflower were concluded as follows： extraction temperature was at 94.71 ℃, extraction time was 1.67 hours, ratio of 22.04 mL·g-1, extraction times was 2, the yield of the polysaccharides was 2.789%, and the test result of experiment of polysaccharides was similar. Conclusion This extraction technology could be used for the extraction of safflower.