响应曲面法优化黄芩苷精制工艺研究

Study on Optimization of Experimental Conditions of Baicalin Purification Technique by Response Surface Methodology

目的:以黄芩苷粗品为原料,利用响应曲面法优化黄芩苷的精制工艺以得到较高纯度的黄芩苷。方法:在单因素实验的基础上,应用Box-Behnken设计方法建立数学模型,选择乙醇浓度、pH值、保温时间和保温温度为影响因子,以黄芩苷纯度为响应值,进行响应曲面分析。结果:确定黄芩苷的最佳精制工艺条件为:乙醇浓度50%,pH值2.00,保温温度80℃,保温时间45min。在最佳精制工艺条件下黄芩苷的平均纯度为91.13%。结论:精制工艺与模型高度拟合,可以用于工业生产。

Objective： In order to get higher purity baicalin from Baicalin crude, Response Surface Methodology（RSM） is employed to optimize experimental conditions of baicalin purification. Methods： Based on the single factor experiments, Box-Behnken design was applied to establish the mathematical model. The optimum purification process was investigated by response surface methodology with ethanol concentration, pH value, holding temperature and holding time as influencing factors, and the product purity of baicalin was considered as index. Results： The results of RSM suggested the best experimental conditions were ： ethanol concentration 50%, pH value 2. 00, holding temperature 80 ℃, holding time 45 min. The average product purity of baicalin was 91.13% under above conditions. Conclusion： The optimum purification process was in good agreement with the predicted counterpart, and can be used for industrial production