环孢素A对大鼠缺血缺氧后酸碱平衡的调节作用

Effects of cyclosporin A on acid-base balance after hypoxia-ischemia in rats

目的探讨不同剂量环孢素A(CsA)对休克引起大鼠缺血缺氧状态下血气指标的影响,初步探讨CsA的抗休克效果。方法采用SD大鼠创伤失血性休克模型,实验动物分为正常对照组、休克对照组、LR液复苏组、LR液+CsA 1 mg/kg组、LR液+CsA 5 mg/kg组、LR液+CsA 10 mg/kg组。各组分别在休克前、休克末、复苏后1 h、复苏后2 h取血,观察pH、HCO3-、碱剩余、乳酸指标的变化情况。结果各组在休克末均出现pH值下降、HCO3-明显降低、BE负值增大、乳酸升高,呈现明显的酸中毒。经过液体复苏后,血气指标有所改善。各剂量的CsA输注均可改善休克后的酸中毒状态,效果明显优于单用乳酸林格氏液复苏,其中以CsA 5 mg/kg的治疗效果最为明显。结论环孢素A对严重创伤失血性休克大鼠血气指标有较好的改善作用。

Objective To investigate the effects of different doses of cyclosporin A （CsA） on acid-base balance after hypoxia-ischemia in rats and discuss the effects of CsA in terms of antishock. Methods The experimental rats were divied into the following 6 groups with traumatic hemorrhagic shuck model of SD rats ： normal control group, shock control group, LR group, LR ＋ CsA 1 mg/kg group, LR ＋ CsA 5 mg/kg group, and LR ＋ CsA 10 mg/kg group. Draw blood of every group before the shock, at the end of shock, 1 hour after recovery, and 2 hours after recovery respectively, and observe the changes of blood pH, HCO3 -, base excess （ BE）, and lactate. Results At the end of shock, blood pH,HCO3 - and base excess decreased while lactate increased obviously which indicated acidosis clearly in all groups. After fluid resuscitation, blood gas index improved accordingly. And the three doses of CsA could relieve symptoms of acidosis with better effects than lactated finger＇ s solution alone. Among them, CsA 5 mg/kg had the best resuscitation effect. Conclusion CsA can effectively improve the blood gases for traumatic hemorrhagic shock.