摘要
目的探讨不同浓度磷脂酰肌醇3激酶(PI3K)抑制剂(LY-294002)对人宫颈癌细胞株Caspase-3表达的影响。方法培养人宫颈癌Hela细胞,随机分为对照组和LY294002干预组,干预组再随机细分为4个亚组,分别以终浓度为5、10、20、40μmol/L的PI3K抑制剂LY294002处理人宫颈癌Hela细胞,作用48 h。Western blot方法和RT-PCR方法检测各组人宫颈癌细胞株Caspase-3表达变化。结果 Western blot方法检测发现,对照组宫颈癌细胞株有微量的Caspase-3阳性表达,在以终浓度为5μmol/L的LY294002处理48h后,宫颈癌细胞株的Caspase-3阳性表达显著升高,与对照组相比差异显著(P<0.05),随着LY294002的终浓度的升高,宫颈癌细胞株的Caspase-3阳性表达也随之上升,呈剂量依赖性。RT-PCR方法检测Caspase-3 mRNA时发现,Caspase-3 mRNA的表达也随着LY294002的终浓度的升高而升高,呈一定的剂量依赖性。结论LY-294002能够上调人宫颈癌细胞Caspase-3的表达。
Objective To study the expression change of Caspase-3 in cervical cancer cell lines induced by LY294002.Methods Hela cells were cultured,and divided into the control groups（treated with dimethyl sulfoxide） and the experiment group（treated with LY294002 for 48 h）.The experiment group was divide into four subgroup according to final concentration of LY294002（5 μmol/L,10 μmol/L,20 μmol/L,40 μmol/L）.The expression of Caspase-3 was examined in Hela cells by western blot methods,and the expression of Caspase-3 mRNA was examined in Hela cells by RT-PCR methods.Result There is a little positive expression of Caspase-3 in Hela cells of control group.Compared with control group,the expression of Caspase-3 in Hela cells of experiment group increased signicantly after treated with LY294002（5 μmol/L）,and the expression of Caspase-3 was increased by LY294002 in some dose dependent.Compared with control group,the expression of Caspase-3 mRNA in Hela cells of experiment group increased signicantly,and the expression of Caspase-3 mRNA was increased by LY294002 in some dose dependent too.Conclusion LY294002 could up-regulate the expression of Caspase-3 in cervical cancer cell lines.
出处
《解剖学研究》
CAS
2013年第1期29-31,39,共4页
Anatomy Research
