大花萱草‘盛夏红酒’组织培养技术研究

Study on Tissue Culture Technique of Hemerocallis middendorffii 'Summer Wine'

[目的]研究大花萱草‘盛夏红酒’的组织培养技术。[方法]以‘盛夏红酒’的健壮花梗为外植体,研究不同激素配比及浓度对外植体愈伤组织诱导和生根的影响。[结果]0.1%升汞消毒10 min为最佳外植体消毒时间,成活率达82.22%;愈伤组织诱导的最佳培养基为MS+2.0 mg/L 6-BA+0.2 mg/L IBA;最佳增殖培养基为MS+1.5 mg/L 6-BA+0.2 mg/L IBA,繁殖系数达4.9;最佳生根培养基为1/2MS+0.5 mg/L NAA,生根率达92.5%;最适移栽基质为蛭石∶草炭(V/V)=1∶1,苗成活率可达95%。[结论]该研究为‘盛夏红酒’的工厂化育苗提供了理论依据。

[Objective] The paper was to study the tissue culture technique of Hemerocallis middendorffii ’Summer Wine’.[Method] The effects of hormones with different ratios and concentrations on callus induction and rooting were studied by using the robust pedicel of ’Summer Wine’ as explants.[Result] 0.1% HgCl2 disinfection for 10 min was the best sterilization time of explants,and the survival rate was up to 82.22%.The best medium for callus induction was MS＋2.0 mg/L 6-BA＋0.2 mg/L IBA.The best medium for proliferation was MS＋1.5 mg/L 6-BA＋0.2 mg/L IBA,and the propagation coefficient was 4.9.The best rooting medium was 1/2MS＋0.5 mg/L NAA,and the rooting rate was up to 92.5%.The suitable transplanting medium was vermiculite： turf（V/V）=1∶1,and the seedlings survival rate reached 95%.[Conclusion] The study provided a theoretical basis for the industrial seedling of H.middendorffii ’Summer Wine’.