苜蓿MtCDPK1基因序列分析、克隆及表达载体构建

Cloning,Sequence Analysis and Expression Vector Construction of Medicago MtCDPK1 Gene

[目的]克隆苜蓿MtCDPK1基因,对其进行序列分析,并构建其表达载体。[方法]根据MtCDPK1基因的全长序列设计引物来克隆该基因,并用生物信息学方法对该基因表达的蛋白序列进行分析,最后通过酶切、连接、转化构建该基因的表达载体。[结果]试验成功克隆到了MtCDPK1基因,并证明MtCDPK1蛋白属于Ca2+依赖的蛋白激酶,同时成功构建了该基因的表达载体。[结论]该研究为苜蓿的遗传转化提供了良好的基础。

[Objective] The paper was to clone and analyze the sequence of Medicago MtCDPK1 gene,and to construct its expression vector.[Method] Primers were designed according to the sequence of MtCDPK1 to clone the gene,and the protein sequence was analyzed by bioinformatics methods.Finally,the expression vector of the gene was constructed by enzyme digestion,connection and transformation.[Result] The MtCDPK1 gene was successfully cloned and it showed that the MtCDPK1 protein belonged to the Ca2＋-dependent protein kinase.The expression vector of the gene was also constructed successfully.[Conclusion] The study provided a good foundation for the genetic transformation of Medicago.