金线莲白绢病病原菌分离及分子鉴定

Isolation and Molecular Identification of Southern Blight Pathogen in Anoectochilus roxburghii(Wall.)Lindl.

采用组织块法对金线莲疑似白绢病病原菌进行分离,用致病性试验、病原菌形态学观察、分子生物学方法对该病原菌进行鉴定。结果表明:从收集的福建闽南地区金线莲疑似白绢病感染植株中分离得到菌株,菌丝白色,绒状,紧贴培养基,向四周呈辐射状扩展,有隔及分枝;菌落表面形成菌核,菌核初呈白色,后变黄色,最后呈茶褐色,球形或不规则球形,表面光滑有光泽;分离的菌株可使金线莲产生病害,对扩增得到的rDNA-ITS序列进行Blast对比分析,确定该金线莲病害为罗氏小核菌Sclero-tiumrolfsii Sacc.引起的白绢病。

In this study, the suspected Pathogen of Anoectochilus roxburghii Southern blight was isolated by tissue separation and purified from samples with typical symptoms. Then the pathogen was identified by pathogenicity test, pathogen morphology and molecular biology methods. The results showed that the strain from southern blight infected Anoectochilus tissues collected from Fuiian were isolated. Mycelium was white, velvet-like, close to the culture medium, radially extended to the surrounding containment and branches, colonies formed on the surface of sclerotia, sclerotia early white, then change to yellow, and finally was dark brown, spherical or irregular spherical, smooth and shiny surface. The isolated strain has pathogenicity to tested Anoectochilus. The pathogen was identified pathogenically, morphologically and molecularly. Based on these data, it can be confirmed that the pathogen was Sclerotium rolfsii Sacc.