NaCl胁迫对野生与栽培大豆杂交后代株系生理指标的影响

Effects of NaCl Stress on Physiological Characters of Soybean Hybrids from Glycine max×Glycine soja

为筛选耐盐性强的大豆材料,设置不同NaCl溶液浓度梯度(50,100,150和200 mmol.L-1)模拟盐渍环境,对野生大豆与栽培大豆杂交后代15个稳定株系、父本野生大豆YDD和当地栽培大豆对照KYDD的幼苗叶片进行保护酶活性、丙二醛(MDA)含量和渗透调节物质含量进行测定。结果表明:随着NaCl浓度的增加,所有株系丙二醛含量明显增加,超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)活性都有不同程度的降低,可溶性蛋白(SP)受到分解且含量逐渐降低,游离脯氨酸(Pro)含量略有增加,可溶性糖(SS)含量极低且增幅不明显。在不同浓度NaCl胁迫下ZS、2号和15-1的保护酶活性明显高于YDD和KYDD;只有15-1的丙二醛、游离脯氨酸和可溶性糖含量低于YDD和KYDD;而15-1的可溶性蛋白含量高于YDD和KYDD,所以15-1是耐盐性最强的株系。

Seedlings of fifteen stable soybean lines from Glycine max x Glycine soja,as well as male parent YDD（ Glycine soja） and local control cultivar KYDD （Glycine max）, were exposed to 0,50,100,150 and 200 mmol·L^-1 NaC1, and protective en- zyme activity, content of Malondialdehyde（MDA） and osmotic regulation substances were determined to screen soybean materi- als with higher salt tolerance. With the increment of NaC1 concentration, MDA content significantly increased, activities of su- peroxide dismutase （ SOD）, peroxidase （POD） and catalase （CAT） decreased with varied degree, soluble protein （SP） declined, free proline （Pro）slightly increased, soluble sugar（SS） content was lower and remained stable. Under different concentrations of NaC1,SOD,POD and CAT activities of ZS,2 and 15-1 were obviously higher than YDD and KYDD,whlie MDA,Pro and SS were lower,and SP were higher thanYDD and KYDD for 15-1. Hence, 15-1 had best salt tolerance from tested soybean lines.