振动刺激对SMDA大鼠肌肉中Myf-5含量的影响

Effects of Vibration Stimulate on Myf-5 in SMDA Rats Muscle with Disuse Atrophy

目的:探讨振动刺激对大鼠腓肠肌中Myf-5含量的影响,进而说明振动刺激对废用性萎缩肌肉产生的作用。方法:将40只SD雌性大鼠随机分为正常对照A组、低频振动B组、高频振动C组、悬吊D组、自然恢复E组,共5组。除A组外其他4组同时悬吊14天后,取A、D、E3组大鼠的腓肠肌肌肉组织,对B、C两组分别进行低频、高频振动刺激2周后,再取其大鼠腓肠肌肌肉组织,采用Western Blotting印迹法对各组的Myf-5进行定量分析并进行比较。结果:D组Myf-5表达明显下降(P<0.05),B、C两组Myf-5表达显著增加(P<0.05)。结论:20Hz和30Hz的振动刺激干预可以有效提高肌肉Myf-5表达水平;低频振动干预(20Hz)较高频振动干预(30Hz)的振动刺激对SMDA骨骼肌效果更明显。

Objective： To study the effects on Myf-5 content in rats gastrocnemius muscles by vibrate stimuli,which express the role of the SMDA by the Vibration stimulation further.Methods： 40 Female SD rats were assigned randomly to one of the five groups： simultaneous control（A）,Suspension plus low frequency vibration stimulate（B）,Suspension plus high frequency vibration stimulate（C）,Suspension（D）,and Suspension control（E）.gastrocnemius muscles of Group A were examined 14 days later,and other 4 groups were examined after 2 weeks’vibration stimuli.The change of Myf-5 was measured by Western blotting.Results： Compared with that of group A,the expression of Myf-5 in group D was significantly reduced（P ＆lt; 0.05）.Compared with group D,the expression of Myf-5 recovered completely in group B＆C（P ＆lt; 0.05）.Conclusion： 20Hz and 30Hz vibration stimulation intervention can improve muscle Myf-5 expression levels,and low frequency vibration training（20Hz） is more obvious stimulus effects than high frequency vibration training（30Hz）.