摘要
目的:检测尿苷二磷酸葡萄糖醛酸基转移酶(UGT)1A6 A541G基因多态性在黑龙江汉族癫痫患者中的分布频率,探讨UGT1A6基因多态性对丙戊酸血药浓度的影响。方法:应用荧光偏振免疫法(FPIA)测定患者丙戊酸钠的血药浓度,PCRRFLP技术检测患者UGT1A6 541位点的基因多态性,PCR扩增产物进行测序验证方法的可靠性。结果:97例汉族癫痫患者中有28名(28.9%)携带突变型UGT1A6基因,其中服用单位剂量(mg·kg^(-1))引起的血药浓度AA基因型患者为(3.06±0.80)μg·ml^(-1),AG基因型患者为(2.21±0.66)μg·ml^(-1),两者差异有统计学意义(P<0.05)。结论:UGT1 A6基因多态性对丙戊酸血药浓度有影响,临床上对于含突变型UGT1A6基因的患者服用丙戊酸时应较常规减少用药减量。
Objective：To investigate the distribution and frequency of UGT1A6 A541G genetic polymorphism in Han epileptic patients from Heilongjiang,and evaluate the effect of UGT1 A6 A541G genetic polymorphism on the serum concentration of valproic acid. Method：Fluorescence polarization immunoassay（FPIA） was used to measure the serum concentration of valproic acid and the results were standardized by dosage and body weight.UGT1A6 A541G genetic polymorphism was detected by PCR-RFLP.Direct sequencing was used to confirm the expected sequences of each genotype.Result;Among the 97 cases,28（28.9%） were with the A/G genotypes. The mean values of serum concentration of valproic acid in the patients with A/A genotype was（3.06±0.80）μg·ml-1,significantly higher than that in the patients with A/G genotype（2.21±0.66μg·ml-1,P＆lt;0.05）.Conclusion;The genetic polymorphisms of UGT1A6 gene affect the metabolism of valproic acid.The dosage of sodium valproate for the patients with 541G allele in UGT1 A6 should be less than the usual.
出处
《中国药师》
CAS
2013年第6期802-804,共3页
China Pharmacist
