HPLC法测定养阴清肺丸中5-羟甲基糠醛的含量

Determination of 5-Hydroxymethylfurfural in Yangyin Qingfei Pills by HPLC

目的:建立养阴清肺丸中5-羟甲基糠醛含量的测定方法,考察不同企业养阴清肺丸中5-羟甲基糠醛的含量差异。方法:采用高效液相色谱法。色谱柱为Agilent C_(18)(250 mm×4.6 mm,5μm)柱,柱温为30℃,流动相为甲醇-水(10:90),流速0.8 ml·min^(-1),检测波长:284 nm,进样量为10μl。结果:5-羟甲基糠醛进样量在0.020 2～0.404 0μg范围内与峰面积积分值呈良好的线性关系(r=1.000 0);平均加样回收率为101.52%,RSD=2.7%(n=6)。结论:该方法简便、准确,灵敏度高、重复性好,可用于养阴清肺丸中5-羟甲基糠醛的含量测定。

Objective：To establish a method for the content determination of 5-hydroxymethylfurfural in Yangyin Qingfei pills,and study the content difference in Yangyin Qingfei pills from different enterprises.Method：The determination was performed on an Agilent C18（250 mm×4.6 mm,5μm） column at 30℃with the mobile phase consisted of methanol-water（10：90） at the flow rate of 0.8 ml·min-1.The detection wavelength was set at 284nm and the injection volume was 10μl.Result;The linear range of 5-hydroxymethylfurfural was 0.020 2-0.404 0μg（r = 1.000 0） with the average recovery of 101.52%（RSD = 2.7%,n = 6）.Conclusion：The method is simple,accurate,sensitive and reproducible in the content determination of 5 - hvdroxymethylfiirfurai in Yangyin Qingfei pills.