miR-183靶向Ezrin表达对肝癌细胞侵袭和转移的抑制作用

Targeted-Ezrin expression by miR-183 in inhibition of hepatoma cell invasion and metastasis

目的 :探讨miR-183靶向Ezrin表达对肝癌细胞侵袭转移能力的影响。方法 :培养肝癌细胞(HepG2)和正常肝细胞(LO-2),以逆转录实时定量PCR和Western Blot分别检测miR-183和Ezrin蛋白相对表达;合成miR-183模拟物转染HepG2细胞,观察转染前、后Ezrin蛋白表达;以划痕和transwell试验比较转染前、后肝癌细胞迁移侵袭能力的变化。结果:与LO-2细胞相比,miR-183在HepG2细胞低表达(P=0.023),靶基因Ezrin蛋白高表达(P=0.033)。转染miR-183模拟物后,HepG2细胞中Ezrin蛋白的表达明显下降(P<0.001)且细胞迁移侵袭能力也显著降低。结论:miR-183为监测肝癌转移的潜在标志并经负调控Ezrin抑制细胞侵袭转移。

Objective： Targeting Ezrin by miR-183 to explore the effects on invasion and metastasis of hepatoma cells.Methods： HepG2 and LO-2 cells were cultured.The relative expression levels of the miR-183 and Ezrin protein were quantitated by qRT-PCR and Western Blot in both cell lines.The alteration of Ezrin levels in HepG2 cells were observed before and after miR-183 mimics transfected.The migration and invasion of HepG2 cells were examined in vivo by the wound healing or transwell assay.Results： Relative quantification showed lower expression level of miR-183（P=0.023） and higher level of Ezrin protein（P=0.033） in HepG2 cells than that in LO-2.However,down-regulation of Ezrin by miR-183 was found after mimics transfected in HepG2 cells（P0.001）,and that significantly inhibited the cells migration and invasion in vivo.Conclusion： The miR-183 is a potential molecular marker for monitoring hepatoma cells metastasis and inhibits the invasion and metastasis of hepatoma cells through the down-regulation of Ezrin by miR-183.