陆地棉LTP家族基因的克隆与表达分析

Cloning and Expression Pattern Analysis of Gossypium hirsutum Lipid Transfer Protein Gene Family

长链脂肪酸能够通过调节胚珠内源乙烯信号变化来促进棉花的起始和伸长发育,为深入了解棉花脂肪酸的转运过程及参与基因,对棉花全基因组进行了分析,克隆了11个脂质转运蛋白。实时荧光定量PCR证实:LTP6,LTP7,LTP9,LTP11 4个基因在纤维起始时期(野生型与突变体之间)的表达存在差异,这4个基因可能与纤维起始发育有关。通过基因组步移技术克隆了Gh-LTP6基因的启动子,并对其结构进行分析,发现其具有多个MYB结合位点。这些结果为深入研究LTP基因在纤维起始过程中的角色奠定了基础。

Long-chain fatty acids could activate cotton fiber initiation and elongation by regulating intracellular ethylene signal transduction in cotton ovule. In order to fully understand how fatty acids are transferred to the surface of fiber cells and how many genes are involved in this process, we cloned 11 LTP genes after genome-wide search and analyzed their expression patterns during fiber＇s development by quantitative RT-PCR. The results showed that there are differences between expressions ofLTP6, LTP7, LTP9 and LTPll, and these 4 genes might be closely related to cotton fiber development at the original stage. We also cloned Gh-LTP6 promoter by Genome Walking technique and analyzed its motif, results showed there are mutiple MYB binding site. These results might lay a solid foundation for intensive studying the role of LTP genes in the process of cotton fiber development.