环介导恒温扩增技术在植物病原物检测中的应用

Application of Loop-mediated Isothermal Amplification in Detecting Plant Pathogens

环介导恒温扩增反应(loop-mediated isothermal amplification,LAMP)是一种新型的核酸扩增方法,具有特异性强、灵敏度高、操作简单且适合于多种检测环境等优点,自开发以来,其作为一种分子生物学检测技术,被广泛应用于许多领域。简要介绍了该技术的原理,综述了其在植物病原物检测方面的应用,讨论了其在应用中出现的问题,并针对这些问题提出了相应的解决办法,最后展望了其应用前景。

Loop-mediated isothermal amplification （LAMP） was a novel method for nucleic acid amplification with the advantages of high specificity, high sensitivity, simple operation, and fitness for detection of varieties environments, and had been widely used in many fields as a molecular biological detection technology. This paper briefly introduced the principle of LAMP, reviewed its application in detecting plant pathogens, discussed the problems occurring in its applicatioh and provided relavent solutions according to these problems, and finally outlooked its application prospect.