大鼠左心室插管术的改进方法及心气虚证模型大鼠左心功能指标的测定

Improved Method of Left Ventricular Cannulation and Measurement of Cardiac Function Parameters in Model Rats With the Heart-Qi Deficiency Syndrome

目的:观察采用改进的大鼠左心室插管术制作心气虚证大鼠模型的可行性。方法:40只雄性SD大鼠随机分成2组,空白对照组和模型组。采用控食、负重强迫游泳及大剂量普萘洛尔等复合因素建立SD大鼠心气虚证模型,RM6240BD多道生物信号采集处理系统检测心功能相关指标。结果:①经改进大鼠左心室插管术,成功率明显提高。②与空白对照组比较,模型组大鼠最大心室内压、左心室收缩压(LVSP)、平均心室内压、心率(HR)等指标均降低(P<0.01),左心室内压最大上升速率(+dp/dtma)x、左心室内压最大下降速率(-dp/dtma)x及t-dp/dtmax均升高(P<0.05,P<0.01)。结论:大鼠左心室插管术的改进方法成功率提高,心气虚证模型大鼠心功能指标改变明显。

Objective：To observe feasibility of modeling method,which heart-Qi deficiency syndrome model rats were established by improved left ventricular cannulation.Methods：40 male SD rats were randomly divided into control group and model group.Heart-Qi deficiency syndrome model rats were established through alimentary control,load swimming combined with large dose propranolol.Cardiac function parameters of rats were detected by RM6240BD multiple biological signal collecting and processing system.Results：①The success rate of operation was apparently elevated by improving the skill of left ventricular cannulation.②Compared with control group,maximum ventricular pressure,left ventricular systolic pressure（LVSP）,average ventricular pressure,heart rate（HR）of rats in model group were decreased（P0.01）,left heart indoor pressure maximum climbing speed（＋dp/dtmax）,left heart indoor pressure maximum decline rate（-dp/dtmax）and t-dp/dtmax of rats in model group was increased（P0.05,P0.01）.Conclusion：The success rate of modeling is increased by the improved skill of left ventricular cannulation,and the indexes of cardiac function are changed apparently.