奠基方含药血清对类多囊卵巢综合征大鼠卵巢颗粒细胞分泌功能影响的研究

Effect of Serum Contained Dianji Decoction on the Secretion Function of Ovarian Granulosa Cells From PCOS Rats

目的:观察奠基方含药血清对类多囊卵巢综合征(PCOS)大鼠卵巢颗粒细胞(GC)体外分泌功能的影响。方法:采用血清药理学方法,以不同类PCOS大鼠含药血清,与体外培养的颗粒细胞共同培养48 h,观察奠基方含药血清对类PCOS卵巢颗粒细胞雌激素(E2)、孕酮(P)分泌量的影响。结果:奠基方含药血清可明显增加颗粒细胞E2的分泌量,降低P的分泌量。结论:奠基方可通过调节颗粒细胞的分泌功能进而改善类PCOS的卵巢功能。

Objective：To observe the effect of serum contained Dianji Decoction on the secretion function of ovarian granulosa cells from polycystic ovary syndrome（PCOS）rat.Methods：The different kinds of the serum with decoction from PCOS rat was added to cultured granulosa cells and incubated for 48 h.Then the concentration of estradiol（E2）,progesterone（P）in cultured medium were measured by Sandwich ELISA respectively.Results：The serum contained Dianji Decoction could increase the secretion of E2 and debase P.Conclusion：Dianji Decoction can improve the ovarian function of PCOS rat by regulating the secretion function of granulosa cells.