摘要
为探讨冷冻温度对牛精子复苏规律的影响,本研究利用全自动细管精液程控冷冻仪软件编制冷冻程序,控制冷冻温度变化,测定精液在不同冷冻温度曲线下冷冻精子复苏规律变化。结果表明,冷冻温度曲线按照:5～-10℃、降温速率5℃/min,-10～-100℃、降温速率35℃/min,-100～-140℃、降温速率20℃/min冷冻程序曲线变化,精子复苏率较高,能达到71%。对冷冻温度曲线进行优化选择,可以提高精液冷冻的复苏率。
In order to explore the impact of the freezing temperature of frozen bovine semen spermatozoa re- covery law. The preparation of program-controlled automatic microtube semen freezing instrument software frozen program to control the the semen freezing temperature change measured semen frozen in different freez- ing temperature curve sperm recovery rate variation. The results showed that when the freezing temperature curve in accordance with ＋5 to -10 ℃, the cooling velocity for 3 ℃ / min, from -10 to -100 ℃, the cooling ve- locity for 38.6 ℃/min, from -100 to -130 ℃, the cooling velocity for 20 ℃ / min, The percentage of anabiosis is high, up to 71%. To optimize the semen freezing temperature curve, you can improve the success rate of the se- men frozen.
出处
《现代畜牧兽医》
2013年第6期51-53,共3页
Modern Journal of Animal Husbandry and Veterinary Medicine
关键词
精液
冷冻温度曲线
复苏率
semen
freezing temperature curve
percentage of anabiosis
