猪苓介导AQP1和AQP3在治疗膀胱癌中作用

The Regulation of Sclerotia of P. Umbellate Aqueous Extract with Bladder AQP1,AQP3 in Urinary Bladder Cancer

目的观察利水渗湿中药猪苓对膀胱癌大鼠膀胱组织中AQP1和AQP3表达的调节作用。方法以猪苓干预BBN联合糖精诱导Fisher-344大鼠膀胱癌模型,使用免疫组化和RT-PCR技术测定膀胱癌组织中AQP1、AQP3的表达。结果与模型组比较,猪苓250mg/kg和500mg/kg剂量对大鼠膀胱癌模型膀胱组织AQP1、AQP3mRNA相对表达含量显著下调。结论猪苓利水渗湿功效可能通过调节膀胱癌组织AQP1和AQP3产生抗膀胱癌作用。

Objective To evaluate regulation of the AQP1,AQP3 in bladder post oral administration of a diuressis-reemoving dampness chinese medieine,sclerotia of P. umbellate aqueous extract in urinary bladder cancer rats. Methods The sclerotia of P. umbellate aqueous extract given Fisher-344 rats induced by N-butyl-N-（4-hydroxybutyI）nitrosamine （BBN）combined saccharin, then the regulation of AQP1,AQP3 measured with immunohistoehemieal and RT-PCR. Results The AQPI and AQP3 mRNA relative expressions in bladder carcinogenesis rat bladder post 250mg/kg,500mg/kg selerotia of P. umbellate aqueous extract doses treatment were down-regulated compared to BBN group. Conclusion Inhibition of bladder carcinogenesis by sclerotia of P. umbellate aqueous extract associated with regulation of AQP1 and AQP3 in bladder.