NOMO1基因在大鼠胚胎心脏发育过程中的表达

Expression of nodal modulator 1 gene during development of rat embryonic hearts

目的:检测NOMO1基因在SD大鼠胚胎心脏发育过程中mRNA表达变化及定位,并探讨其在胚胎心脏发育的意义。方法:24只孕鼠随机分为6组,每组4只,分别于妊娠12 d(D12)、15 d(D15)、16 d(D16)、17 d(D17)、19 d(D19)、21 d(D21)时取出胎鼠,取其心脏,采用实时定量PCR(real-time PCR)技术检测心脏组织中NOMO1基因mRNA的表达丰度,并应用荧光原位杂交(FISH)的方法对其进行定位分析。结果:在妊娠D12、D15、D16、D17、D19、D21的胚胎心脏中NOMO1基因mRNA均有表达,且呈由弱到强再减弱趋势,表达高峰出现在D16;该基因主要表达于心外膜中,而在心肌层、心内膜、心瓣膜等处未见表达。结论:NOMO1基因的表达随大鼠胚胎的心脏发育呈动态表达,可能与心脏发育相关。

Objective：To investigate the change of nodal modator 1（NOMO1） gene mRNA expression and its location during the development of rat embryonic hearts,and to explore its significance in embryonic heart development.Methods：Twenty-four pregnant Sprague-Dawley rats were randomly divided into 6 groups（n=4）,embryonic hearts were obtained on the 12th,15th,16th,17th,19th,21th day in pregnancy.Real-time quantitative reverse transcription polymerase chain reaction（real-time PCR） was used to reveal NOMO1 gene mRNA expression,and fluorescence in situ hybridization（FISH） was used for its positioning analysis.Results：With the development of rat embryonic hearts,the expression levels of NOMO1 gene mRNA were from weak to strong signs of abating and its peak was on 16th day.NOMO1 gene was mainly expressed in the epicardium,while it was not detected in the myocardium,endocardium and heart valves.Conclusion：The expression of NOMO1 gene is dynamic,and it may play an important role in the fetal heart development.