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大肠杆菌工程菌高密度发酵的初步研究

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摘要 在5L发酵罐上以恒pH-补料发酵模式为基础,通过改变培养温度、时间以及补料浓度达到提高大肠杆菌产量的目的。实验结果表明,当恒pH值为6.5,补料类型甘油速度为35mL/h。连续补料6h,发酵12h结束,可使湿菌体量达到51.1g/L,初步达到了高密度发酵的要求。
作者 赵雨 倪孟祥
出处 《山东化工》 CAS 2013年第6期23-25,34,共4页 Shandong Chemical Industry
基金 前国家医药管理局资助项目
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