固定化β-葡萄糖苷酶填充床反应器的研究

Studies on the Packed-bed Reactor of Immobilized β-Glucosidase

以多孔陶瓷球为载体,戊二醛为交联剂,制备了固定化β-葡萄糖苷酶,将固定化酶装填于填充床反应器中处理纤维二糖-葡萄糖混合糖液,考察固定化酶连续酶解纤维二糖的条件和使用稳定性。实验结果表明:反应器酶解混合糖液的最适pH值4.8、温度60℃;随着底物进料流速的增加,转化率逐渐下降,而体积生产效率不断提高;当进料流速在5.47 mL/min时,反应器转化率为91.7%,体积生产效率为8.98 g/(L.h),在此流速下,反应器连续酶解10 d,流速稳定,转化率始终维持在90%以上,平均转化率为90.7%。

The β-glucosidase was immobilized on the carrier of porous ceramic balls with glutaraldehyde by crosslinking reaction. The solution composed by a certain concentration of cellubiose and glucose was continuously treated by immobilized β-glucosidase loaded on a packed bed reactor. The operation condition and stability of this immobilized enzyme during enzymatic hydrolysis were investigated. The results indicated that the optimum operation pH value and temperature were 4.8 and 60 ℃, respectively, in the continuous hydrolysis process. Furthermore, the conversion declined and the production efficiency on volume increased with the rise of flow rate. The conversion rate was 91.7 % as the substrate flow rate and production efficiency on volume were 5.47 mL/min and 8.98 g/（ L. h）, respectively. The mixed cellubiose and glucose solution was continuously enzymatic hydro- lyzed for 10 clays under this stable flow rate at 5.47 mL/min. The conversion rate of cellubiose could be maintained at a constant lever higher than 90 %, and the mean conversion rate was 90.7 % in present study.