摘要
在121℃下进行稀酸(0.5%,1.0%,1.5%和2.0%)预处理泡桐30~90 min,检测水解液中的总还原糖、各种单糖的浓度以及预处理过程中抑制物(糠醛和羟甲基糠醛)。在控制抑制物质量浓度在1 g/L以下,即不影响后续酶解发酵的情况下得到良好的酶解效率。最佳预处理条件为稀酸质量分数1.5%,反应时间60 min,该条件下总还原糖的得率可达到理论值的82%,葡萄糖和木糖的转化率分别达到了39.5%和48.7%。
Dilute sulfuric acid pretreatment of paulownia before cellulose enzymatic hydrolysis was investigated. The biomass with a 10 % solid loading rate was pretreated at 121 ℃ by dilute sulfuric acid with different concentrations (0.5 % , 1.0 % , 1.5 % and 2.0 % ) for 30, 60 and 90 min. The concentrations of the total reducing sugars, monosaccharides and inbibitors ( e. g. fur- rural and HMF(5-hydroxymethylfurfural)) in the pre-hydrolyzate were analyzed to obtain the optimum acid hydrolysis condition that did not affecting the flowing enzymatic hydrolysis. The total reducing sugar yield was about 82 % of the theoretical maximum value, and the conversion efficiencies for glucan and xylan were 39.5 % and 48.7 % , respectively, when the biomass was pre- treated by 1.5 % sulfuric acid for 60 min.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2013年第3期110-114,共5页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(20977033)
教育部归侨科学研究基金(无编号)
关键词
生物乙醇
泡桐
稀酸预处理
纤维素酶
bioethanol
paulownia
dilute acid pretreatment
cellulases