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病毒诱导的基因沉默在植物抗病基因功能研究中的应用 被引量：6

Progress in Study of Plant Resistance Gene Function Using Virus Induced Gene Silence System
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摘要 病毒诱导的基因沉默(Virus induced gene silencing,VIGS)是一种植物抵抗病毒侵入的自然机制,现在已被开发成通过插入目的基因片段的重组病毒来抑制植物内源基因表达的遗传技术,主要用于研究目标基因的功能。作为一种新型的基因鉴定和功能研究的技术工具,VIGS具有无需事先知道目的基因全长序列、快速获取表型、无需获得转基因植株等诸多优点,已越来越广泛地被应用于植物基因功能研究领域。本文从VIGS的作用机制、VIGS体系的优点及局限性以及VIGS在植物抗病机制方面的研究进展等几个方面对病毒诱导的基因沉默进行了综述。 Virus induced gene silence（VIGS） is a natural mechanism, which was used by plants to resist the virus invasion. Now, it has been developed into a popular genetic technique to suppress the endogenous gene expression by recombinant viruses which contain the fragment of target genes. As a novel tool to unravel the candidate gene＇s function, VIGS has many advantages such as unnecessarily knowing the full-length sequence of the target gene in advance, quick acquisition of phenotype, and unnecessarily obtaining the transgenic plant. Now, it has been widely used in the field of plant gene function studies. In this review, we summarized the research progress in VIGS mechanism, the advantages and limitations of VIGS system and its application to studying the plant resistance gene＇s function.
作者 张晓萝 赵君
机构地区 内蒙古农业大学
出处 《中国马铃薯》 2013年第3期181-186,共6页 Chinese Potato Journal
基金 国家自然科学基金项目 水杨酸在马铃薯小G蛋白StRac介导的防卫反应体系建立过程中的功能初探(31260425)
关键词 病毒诱导的基因沉默(VIGS) 抗病机制 基因功能 VIGS plant resistance gene function
分类号 S532 [农业科学—作物学]
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参考文献38

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二级参考文献183

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共引文献39

同被引文献65

引证文献6

二级引证文献24

中国马铃薯
2013年 第3期

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