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Marc-145细胞CD163基因克隆及其真核表达质粒构建 被引量：3

Cloning and construction of expression vector of CD163 gene of Marc-145 cell
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摘要 猪繁殖与呼吸综合征是危害养猪业重要传染病之一,CD163是猪繁殖与呼吸综合征感染易感细胞受体之一。从Marc-145细胞克隆出CD163基因并测序,再根据得到序列,应用Primer5.0软件设计引物,成功地扩增出带酶切位点CD163 ORF基因,通过HindⅢ和XhoⅠ双酶切位点将CD163基因克隆入真核表达载体pcDNA3.1-myc-His中,获得重组质粒pcDNA3.1-CD163,并将重组质粒pcDNA3.1-CD163在BHK细胞上进行表达鉴定。结果表明,Marc-145细胞CD163蛋白在BHK细胞中得到了表达。这为进一步研究CD163受体在PRRSV感染Marc-145细胞过程中作用以及与其他受体之间相互作用提供试验数据。 Porcine reproductive and respiratory syndrome virus （PRRSV） has been acknowledged as one of the most important agents affecting swine. The scavenger receptor CD163 is one of the important entry mediators for PRRSV. In order to ascertain the function of CD163 gene, the CD163 gene was cloned from Marc-145 cell by RT-PCR, and then the whole coding frame of CD163 was cloned into the eukaryotic expression vector pcDNA3.1-myc-His. Indirect fluorescence microscopy showed that CD163 were expressed in transfected cells. The results lay foundation for analyzing the function of CD163 in cultured cell.
作者 曹宗喜 焦培荣 林哲敏 于俊勇 吴欣伟 张桂红
机构地区 海南省农业科学院畜牧兽医研究所海南省热带动物繁育与疫病研究重点实验室 华南农业大学农业部兽用疫苗创制重点实验室
出处 《东北农业大学学报》 CAS CSCD 北大核心 2013年第6期28-31,共4页 Journal of Northeast Agricultural University
基金 国家自然科学基金项目(31272564) 国家自然科学基金会(NSFC)-广东联合基金项目(U0931003) 现代农业产业技术体系专项资金项目(CARS-36) 海南省科学事业费项目(琼财预[2013]131号)
关键词 猪繁殖与呼吸综合征病毒 MARC-145细胞 CD163 克隆 表达载体 PRRSV Marc-145 cell CD163 cloning expression vector
分类号 S767.5 [农业科学—森林保护学] X172 [环境科学与工程—环境科学]
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参考文献14

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二级参考文献26

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共引文献4

同被引文献27

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引证文献3

二级引证文献5

东北农业大学学报
2013年 第6期

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