茶陵野生稻冷响应基因OrCr3的克隆及其生物信息学分析

Cloning and in silico Analysis of a Cold Responsive Gene OrCr3 in Chaling Wild Rice (Oryza rufipogon Griff)

OrCr3(Oryza rufipogon cold responsive gene 3)是一个从茶陵野生稻中发现的受低温诱导表达的基因.以茶陵野生稻为材料克隆了其cDNA及其启动子区域序列,生物信息学分析表明,该基因的完整ORF(open reading frame)长为1 110 bp,推测编码一个由369个氨基酸残基组成的山梨糖醇脱氢酶蛋白,理论相对分子质量为39.3 kD,pI值为6.03;山梨糖醇脱氢酶具有催化山梨糖醇向果糖转变的作用;对OrCr3的启动子区域进行分析,发现多种可能与逆境应答相关的顺式作用调控元件;该基因编码的蛋白在不同物种中存在高度相似性.以上结果说明OrCr3基因可能是一个保守的耐逆相关基因.

OrCr3 （Oryza rufipogon cold responsive gene 3）is one of the cold induced genes screened from Chaling wild rice （Oryza rufipogon Griff.） genome. The sequences of its cDNA and the promoter region were cloned using Chaling wild rice as material. In silico analysis showed that the size of its complete ORF（open reading frame） is 1 110 bp, encoding a putative sorbitol dehydrogenase composed of 369 amino acid residues with relative molecular mass of 39.3 kD and pI value of 6.03. Sorbitol dehydrogenase functions as catalytic transformation of sorbitol to fructose. In the promoter region of the OrCr3, a variety of stress responsive cis- acting regulatory elements were found. Additionally, there is a high degree of similarity for its encoding pro- tein in different species. It is concluded from above results that OrCr3 is a putative conserved stresses relat- ed gene.