新疆两冷泉菌藻席细菌组成及多样性分析

Bacterial Composition and Diversity Analysis of Two Cold Spring Microbial Mats in Xinjiang

【目的】通过对含硫冷泉和非含硫冷泉菌席多样性的研究,为开展低温微生物资源的开发利用以及生物修复和活性物质生产提供参考。【方法】利用末端限制性片段长度多态性(Terminal Restriction Fragmentlength polymorphism,T-RFLP),对冷泉菌席多样性进行分析,所获信息与Mica数据库比对,获得各环境下菌席群落组成。【结果】两菌席共包含细菌6门10纲,其中γ-变形菌类群为两个菌席中最为优势类群,分别占乌鲁木齐4号泉和沙湾冷泉菌席的23.71%和40.48%;芽孢杆菌纲类群和梭菌纲类群为各自菌席中第二大类群,分别占22.73%和29.49%;梭菌属(Clostridium)为两菌席共有类群。【结论】菌席群落组成受环境类型影响而差异较大,乌鲁木齐4号泉(含硫冷泉)细菌物种多样性较高。

[ Objective]The aim of this study is to investigate the bacterial composition of microbial mats in sulfur and non -- sulfur containing cold springs. This work maybe shed light on the survey of useful psychrophiles and the exploitation of bioremediations. [ Method]The change of bacterial diversity was detected by Terminal Restriction Fragment Length Polymorphism （ T - RFLP） , and then analyzed by the online Mica database. [ Result] Bacteria in the two microbial mats were classified into 10 classes of 6 phylum, Gammaproteobaeteria was the most dominant group in both mats that account for 23. 7% and 40.48% respectively; Bacilli and Clostridia were the second dominant groups in mat of No. 4 spring and Shawan spring with 22. 73% and 29. 49% coverage respectively; The genera Clostrium was detected in both mats. [ Conclusion] Bacterial composition in microbial mats was mainly affected by their habitats, and Urumqi No. 4 spring has a higher diversity in species than Shawan spring.