牡蛎过敏原Crag1的二级结构及其B细胞和T细胞表位分析

Analysis of the secondary structure, B cell and T cell epitopes of oyster allergen Cra g 1

目的分析牡蛎过敏原Cra g 1的二级结构及其B、T细胞表位,为探索基于抗原表位的免疫治疗奠定基础。方法从Uniprot数据库中获得牡蛎过敏原Cra g 1的氨基酸序列,通过DNA Star Protean软件,采用Garnier-Robson方法和Chou-Fasman方法分析其二级结构;采用Kyte-Doolittle法、Karplus-Schulz法、Emini法、Jameson-Wolf法综合分析Cra g 1主要的B细胞抗原表位。使用SYFPEITHI和NetMHC-Ⅱ在线网站分析T细胞抗原表位。结果牡蛎过敏原Cra g 1的二级结构主要为α-螺旋,其B细胞表位接近覆盖Cra g 1氨基酸全序列;而T细胞优势表位位于第89～103、108～122、129～143位肽段。结论本研究分析牡蛎过敏原Cra g 1的B、T细胞优势表位,为日后Cra g 1的基础免疫学研究,如疫苗的研制、诊断试剂的制备和其抗原性改造等提供了理论依据。

Objective To analyze the secondary structure and B, T cell epitopes of the oyster major allergen Crag 1, develop the immunotherapy of oyster allergy based on antigenic epitopes. Methods The amino acid sequence of oyster major allergen Cra g 1 was acquired from Uniprot database. The secondary structure of Crag 1 was analyzed by DNAStar-Protean software with Gamier-Robson method and Chou-Fasman method. The B cell epitopes of Cra g 1 were analyzed synthetically by Kyte-Doolittle method, Karplus-Schulz method, Emini method and Jameson-Wolf method. The T cell epitopes of Crag 1 were analyzed by SYFPEITHI and NetMHC- Ⅱonline website. Results The analysis of secondary structure showed Cra g 1 was mainly constituted by α-spiral. B cell epitopes covered almost the whole amino acid sequence of Crag 1. However, T cell epitopes analysis demonstrated that the regions of 89-103,108-122 and 129-143 were the dominant T cell epitopes. Conclusion This study, which analyzed the dominant B, T cell epitopes of oyster allergen Cra g 1, provides a theoretical basis for further basic immunology researches of Crag 1,such as vaccine development, diagnosis reagents preparation, and its antigenicity modification.