九味白术汤对实验性溃疡性结肠炎大鼠血清白介素-1β及白介素-10的影响

Effect of Jiuweibaizhutang on serum levels of interleukin -1β and interleukin-10 in ulcerative colitis rats

目的观察九味白术汤(JWBZ)对溃疡性结肠炎(UC)大鼠血清白介素-1β(IL-1β)及白介素-10(IL-10)水平的影响。方法 48只大鼠完全随机分为正常对照组、模型组、柳氮磺胺吡啶(SASP)组、JWBZ高剂量组、中剂量组与低剂量组;采用2,4,6-三硝基苯磺酸(TNBS)复制大鼠溃疡性结肠炎模型;连续给药10天后,观察UC大鼠结肠黏膜损伤指数(CMDI)、血清IL-1β及IL-10水平的变化。多组间比较采用单因素方差分析。结果正常对照组、模型组、JWBZ低、中、高剂量组及SASP组的CMDI分别为:(0.25±0.46)、(3.38±1.06)、(1.63±0.92)、(1.50±0.53)、(2.50±0.93)、(2.38±0.74)分;相应的IL-1β水平分别为:(232.94±21.77)、(756.67±71.70)、(469.57±71.13)、(401.97±39.24)、(598.93±45.34)、(655.66±10.51)ng/L;IL-10水平分别为:(52.14±7.07)、(20.19±5.17)、(56.79±9.47)、(63.96±9.37)、(41.73±10.55)、(51.13±2.81)ng/L。与正常对照组比较,模型组CMDI显著增加(P<0.01),血清IL-1β水平显著升高(P<0.01),而血清IL-10水平显著降低(P<0.01);与模型组比较,JWBZ低、中、高剂量组及SASP组CMDI均显著降低(P<0.05或P<0.01),血清IL-1β水平均显著降低(P<0.05或P<0.01),而血清IL-10水平显著升高(P<0.01);与SASP组比较,JWBZ中剂量组CMDI显著降低(P<0.05),高、中剂量组血清IL-1β水平显著降低(P<0.01);中剂量组血清IL-10水平显著升高(P<0.01),而低剂量组血清IL-10水平显著降低(P<0.05)。结论 JWBZ对UC大鼠结肠损伤具有良好的修复作用,其机制可能通过调节促炎因子与抗炎因子的平衡实现。

Objective To observe the effect of Jiuweibaizhutang （JWBZ） on serum levels of interleukin-1β（IL-1β） and interleukin-10 （IL-10） in ulcerative colitis （UC） rats. Methods 48 rats were randomly divided into 6 groups： norma! group, model group, salazosulfapyridine （SASP） group, low-dose, middle-dose and high-dose JWBZ groups. Ulcerative colitis rat model was copied by 2, 4, 6-TNBS. After 10 days＇treatment, the changes of colonic mucosal damage index （CMDI）, serum levels of IL-1β and IL-10 were observed. Significant differences between groups were tested by one-way analysis of variance （ANOVA）. Results The CMDI of normal group, model group, low-dose, middle-dose and high-dose JWBZ groups were（0.25±0.46）、（3.38±1.06）、（1.63±0.92）、（1.50±0.53）、（2.50±0.93）、（2.38±0.74）scores,respectively; levels of IL-1β were（232.94±21.77）、（756.67±71.70）、（469.57±71.13）、（401.97±39.24）、（598.93±45.34）、（655.66±10.51）ng/L,respectively; and level of IL-10 were（52.14±7.07）、（20.19±5.17）、（56.79±9.47）、（63.96±9.37）、（41.73±10.55）、（51.13±2.81）ng/L,respectively. Compared to normal group, the CMDI of model group was significantly increased （P 〈 0.01）, and the serum IL-1β level of it increased significantly （P 〈 0.01）, while IL-10 level decreased significantly （P 〈 0.01）. Compared to model group, the CMDI of JWBZ and SASP groups decreased significantly （P 〈0.05 or P 〈 O.01）, and the serum IL-II3 level of treatment groups decreased significantly （P 〈 0.05 or P 〈 0.01）, while the IL-10 level increased significantly （P 〈 0.01）. Compared to SASP group, the CMDI of middle-dose JWBZ group significantly decreased （P 〈 0.05）, the serum IL-1β level of high-dose and low-dose JWBZ groups decreased signifi- cantly （P 〈 0.01）, the serum IL-10 level of middle-dose JWBZ group increased significantly （P 〈 0.01）, while it decreased significantly of low-dose JWBZ group （P 〈 0.05）. Conclusion JWBZ treated UC rats effectively by regulating the balance of pro-and anti-inflammatory cytokines.