磷脂酰肌醇3激酶和蛋白激酶B在上皮性卵巢癌组织中的表达及临床意义

The expression and clinical significance of phosphatidylinositol 3-kinase and protein kinase B in the tissue of epithelial ovarian cancer

目的 研究磷脂酰肌醇3激酶（PI3K）和蛋白激酶B（Akt）在上皮性卵巢癌（EOC）组织中的表达和临床意义.方法 运用Western Blot和免疫组化法检测41例EOC（EOC组）、20例正常卵巢（NO,NO组）和20例良性卵巢上皮性肿瘤（BEOT,BEOT组）组织中PI3K和Akt蛋白的表达.结果 PI3K在EOC组中阳性表达率为70.7％（29/41）,显著高于NO组和BEOT组[10.0％（2/20）、20.0％（4/20）],差异有统计学意义（P＜ 0.01）;Akt在EOC组中阳性表达率为73.2％（30/41）,显著高于NO组和BEOT组[10.0％（2/20）、30.0％（6/20）],差异有统计学意义（P＜0.01）.在EOC患者中,临床分期Ⅰ～Ⅱ期PI3K和Akt蛋白的表达与Ⅲ～Ⅳ期比较差异有统计学意义（P＜0.05）；浆液性囊腺癌中PI3K和Akt蛋白的表达与黏液性囊腺癌比较差异无统计学意义（P＞0.05）.结论 PI3K和Akt蛋白在EOC组织中表达显著增强,它们在EOC发生、发展中起到一定的作用,PI3K/Akt通路有望作为抗肿瘤治疗的有效新靶点.

Objective To study the phosphatidylinositol 3-kinase （PI3K） and protein kinase B （Akt） expression and clinical significance in the tissue of epithelial ovarian cancer （EOC）.Methods Western Blot and immunohistochemical staining were applied to investigate the expression of PI3K and Akt in specimens of 41 patients with EOC （EOC group）,20 patients with normal ovary （NO,NO group） and 20 patients with benign epithelial ovarian tumor （BEOT,BEOT group）.Results The positive expression rate of PI3K in EOC group [70.7%（29/41）] was significantly higher than that in NO group and BEOT group [10.0% （2/20）,20.0% （4/20）] （P 〈0.01）.The positive expression rate of Akt in EOC group [73.2% （30/41）] was significantly higher than that in NO group and BEOT group [10.0% （2/20）,30.0% （6/20）] （P〈 0.01）.There was significant difference in the expression of PI3K and Akt between Ⅰ-1Ⅱ stage and Ⅲ-Ⅳ stage in EOC patients （P 〈 0.05）.There was no significant difference in the expression of PI3K and Akt between serous cystadenocarcinoma and mucous cystadenocarcinoma （P 〉 0.05）.Conclusions The expression of PI3K and Akt increases obvioualy in the tissue of EOC.PI3K and Akt may play important roles in carcinogenesis and metastasis of EOC.PI3K/Akt pathway may be served as a potential target for anticancer therapy.