不同光照强度和光质对铁线莲品种繁星(Clematis‘nelly moser’)不定芽诱导和生长的影响

Effects of Different Light Intensity and Light Quality on Adventitious Buds Induction and Growth of Clematis'nelly moser'

以铁线莲品种繁星(Clematis‘nelly moser’)的带节茎段为材料,研究不同光照强度和光质对铁线莲不定芽繁殖的影响.以MS为基本培养基(含有0.2mg/L 1-萘乙酸和2mg/L 6-苄氨基腺嘌呤),设置光照强度梯度为0、500、2 000、3 000和4 000Lux的白光光照;用透明玻璃纸得到蓝(440nm)、绿(560nm)、红(650nm)3种光质,以白光为对照组,强度均为3 000Lux.结果表明:在光照强度为3 000Lux的白光光照下,不定芽的诱导效果最好,其诱导频率和每个外植体上不定芽的数量分别为100%和4.4,每个外植体上不定芽的平均高度为5.66cm,平均节数为4.6个;蓝光能够显著提高不定芽的诱导数量,是相同光强白光诱导数量的1.27倍,蓝光下不定芽SOD和CAT的最大值显著高于其它光质下的数值,且叶绿素a和b的比值最大.

Effects of different light intensity （0, 500, 2 000, 3 000 and 4 000 Lux） and light quality （blue, green and red light） on propagation of axillary buds by Clematis＇nelly moser＇ are studied. MS media with NAA （Naphthaleneacetic Acid） 0.2 mg/L＋6-BA（6-Benzylaminopurine） 2 mg/L are used as basal media. The experimental results show that the 3 000 Lux white light is better for the axillary buds propagation than the other light quality values. Under 3 000 Lux white light, the adventitious buds per explants, average adventitious bud height per explants and average nodes number are 100%, 4.4, 5.66 cm and 4.6, respectively. Besides, it is found that the blue light significantly enhanced the induction efficiency of adventitious buds, which is 1.27 times than that under white light treatments. The physiological and biochemical data show that the activities of superoxide dismutase （SOD）, hydrogen peroxide （CAT）, and the ratio of chlorophyll a to b in adventitious bud under blue light treatment are significantly higher than those in the other light quality treatments.