结球甘蓝小孢子胚诱导与植株再生

Isolated Microspore Culture and Plant Regeneration in Cabbage

以4个结球甘蓝品种为试材,研究不同条件对小孢子胚诱导和植株再生的影响。结果表明:基因型是限制小孢子胚发生的主要因素。适宜取材的花蕾大小为2.5～4.0mm,瓣药比为2/3～7/6,盛花期是取蕾的适宜时期;培养基大量元素减半和添加适量活性炭均可显著提高胚状体诱导率,但对于难出胚基因型没有起到诱导出胚的作用;胚状体的诱导和萌芽需要植物生长调节剂NAA和6-BA的参与,适宜的胚状体诱导培养基为NLN-13+0.2mg.L-16-BA+0.1mg.L-1NAA,胚状体诱导率为38胚/10蕾,适宜的胚状体生芽培养基为MS+0.4mg.L-16-BA+0.1mg.L-1NAA+3%蔗糖+0.7%琼脂,芽诱导率达46.7%。

Different culture conditions affecting microspore derived embryos and plant regeneration were studied with four varieties of cabbage.The results indicated that the genotype was the key factor limiting microspore embryogenesis.Appropriate microspore culture was bud Length 2.5~4.0mm,petal length / anther length 2/3~7/6,and phase of full bloom.Major element in half and appropriate activated charcoal being added to the culture medium could increase the rate of embryo induction,but the genotype inducing hardly embryo had no effect.Embryoid induction and bud required the participation of the plant growth regulator NAA and 6-BA,Appropriate medium of microspore embryogenesis was the NLN-13 ＋ 0.2mg·L-1 6-BA ＋ 0.1mg·L-1NAA,and frequency of embryoid was 38 embryos/10bud-1;Appropriate shooting medium was the MS＋0.4mg·L-16-BA＋0.1mg·L-1 NAA＋ 3% sucrose＋ 0.7% agar,and the shoot induction rate was 46.7%.