转rd29A-ICE1冷诱导基因水稻提高抗寒性研究

Integratting Cold Regulative Gene rd29A-ICE1 into Rice Improves Cold Tolerance

本研究以pCAMBIA1300质粒为基础,构建了植物冷诱导表达载体pCAMBIA1300-rd29A-ICE1,利用农杆菌介导法导入粳稻品种空育131中。PCR、RT-PCR和Southern杂交检测结果表明,ICE1基因已经成功整合到水稻基因组中,并正常表达。与对照相比,低温处理后超表达ICE1基因的水稻转基因株系存活率和脯氨酸含量明显增加,丙二醛含量积累速率明显下降,提高了抗低温胁迫能力。

Based on vector pCAMBIA1300,cold regulative plant expressing vector pCAMBIA1300-rd29A-ICE1 was constructed and transformed into Japonica rice Kongyu131 by Agrobacterium-mediated transgenic technique.PCR,RT-PCR and Southern blot analysis indicated that ICE1 gene had been successfully integrated into the genome of transgenic rice lines and was expressed normally.After low temperature stress treatment,the transgenic lines with high expression levels of the ICE1 gene had obvious higher survival rate and proline content compared to control.In the meantime,the malondialdehyde（MDA） content was much lower.High expression of ICE1 gene improved low temperature stress tolerant capacities in transgenic rice lines.