^(12)C^(6+)重离子诱变海迪茨氏菌及其修复柴油污染土壤的研究

Research on Dietzia Maris Strain Induced by Carbon-Ion Beams and Its Bioremediation of Diesel Oil Contaminated Soil

应用重离子束辐射改良海迪茨氏菌(Dietzia maris)菌株在降解方面的能力,将离子辐射学与环境修复学相结合,论证该菌在以柴油为代表的污染土壤中的修复能力。利用12C6+重离子能量为80MeV、LET为35.5keV.μm-1、吸收剂量率1Gy.min-1、辐射剂量为5～120Gy辐射具有降解石油烃的代表菌株Dietzia maris,经初选、复选筛选得到了突变菌株DMYR9,并对其在构建污染土壤样品中分解柴油率及修复前后土壤系统中酶活性相关参数与化学改良修复前后污染土壤样品相关数据进行对比分析。结果表明,土壤受柴油污染不久后过氧化氢酶、脱氢酶和脂酶的活性呈现上升趋势,由突变菌体DMYR9修复后的污染土壤系统中脂酶活性呈现最低下降趋势,随着土壤中柴油被DMYR9降解,脂酶活性又不断降低,突变菌体数量及柴油降解率都具有很好的正相关性。突变菌株DMYR9进行生物修复的效果优于化学法修复,这对今后在土壤污染治理中开发利用此突变菌体具有指导意义,并具有较高的后续应用利用价值。

The degradation of petroleum hydrocarbons strain were irradiated with 12C6＋ heavy-ion beam with a dose range of 5 to 120 Gy,These ions were accelerated up to 80MeV u-1,and their LET was 35.5keV·μm-1.Primaries and check screening the mutant strain DMYR9,and its decomposition in contaminated soil samples before and after the repair of the activity of the relevant parameters and chemically modified data were analyzed.And there were positive correlations between lipase activity to microbial counts and degradation efficiency of diesel oil.So the soil lipase activity was an excellent indicator of biological activity for monitoring diesel oil decontamination.The results showed that the soil contaminated by diesel,soil catalase,dehydrogenase and lipase activity increased,the lowest lipase activity by mutant bacteria DMYR9 repair contaminated soil system,with the degradation of diesel oil in the soil.Lipase activity and the number of mutations in bacteria is a good positive correlation.The mutant strain DMYR9 has a higher subsequent application value in the treatment and recovery of petroleum hydrocarbon contaminated soil.