应激对大鼠下丘脑、垂体和血浆催产素、加压素和β内啡肽含量的影响

Effect of Stress on the Contents of Oxytocin, Arg-vasopressin and β-endorphin in Hypothalamus, Pituitary and Plasma in Rats

本工作用放射免疫测定法分别测定了大鼠游泳时下丘脑,垂体和血浆内催产素样免疫活性物质(ir-OT)、精氨酸加压素样免疫活性物质(ir-AVP)和β内啡肽样免疫活性物质(ir-β-EP)含量的变化。游泳时,大鼠下丘脑、垂体和血浆内的ir-OT含量明显高于对照组。下丘脑ir-AVP含量也增高,5min,15 min和20 min时显著增加,垂体内ir-AVP含量减少,血浆ir-AVP含量有升高趋势,但无统计学意义。下丘脑和血浆内ir-β-EP含量明显增加,垂体内ir-β-EP显著减少。上述结果表明,游泳应激对大鼠下丘腑,垂体和血浆内OT、AVP和β-EP等神经肽的含量有明显影响。除OT含量增多对游泳不利以外,AVP和β内啡肽含量的变化对游泳应激有何影响,尚待深入研究。

The contents of ir-oxytocin (it-OT), ir-Arg-yasopressin (ir-AVP) and ir-β-endorphin (ir-β-EP) in hypothalamus, pituitary and plasma were measured by. radioimmunoassay (RIA) when the rats were swimming. It was showed that the ir-OT contents in hypothalamus, pituitary and plasma were significantly increased in the animals having swum 1, 5, 15 and 20min (P<0.05-0.01). The ir-AVP content was elevated in hypothalamus (P<0. 05 -0.01), but decreased in pituitary (P<0.05-0.01). Furthermore, a slight increase of ir-AVP content in plasma was observed, with no statistical significance. The ir~β- EP contents in hypothalamus and plasma were significantly elevated (P<0.05-0.01), but it was greatly decreased in pituitary (P<0.05 -0.01). The results suggest that the swimming stress strongly affects the contents of ir-OT, ir-AVP and ir-β-EP in hypothalamus, pituitary and plasma in rats. The effects of AVP and β-EP on swimming activity are unknown except that OT increase has a harmful effect on the rat swimming activity.